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Group by a two-sample t test for independent samples, if the two samples had equal variances. If they had unequal variances, the Mann hitney test was used for evaluation. A P value Nelfinavir (Mesylate) cord (14 m) were cut through the areas of interest, by using a Leica CM1850 cryostat (Leica Mikrosysteme GmbH, Vienna, Austria). To identify human stem cells transplanted into the rat spinal cord, antibodies directed against human nuclei (HuNu) (Chemicon, Temecula, CA, USA) and human mitochondrial marker (MTC02) (Abcam, Cambridge, UK) were used. To follow the fate of the transplanted stem cells and their communication with the host tissue, antibodies directed against nestin, neurospecific enolase (NSE), chondroitin sulfate proteoglycan (NG2), calcitonin generelated peptide (CGRP) (all Millipore); neurofilament 160 (NF160), neurofilament 200 (NF200), glial fibrillary acidic protein (GFAP) (all Sigma, St. Louis, MO, USA); Olig2, choline PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/17139194 acetyltransferase (ChAT), 2,3-cyclic nucleotide 3-phosphodiesterase (CNPase), Ki67 (all Abcam); Islet2, Nkx6.1 (DSHB, Iowa City, IA, USA), and TauAmemori et al. Stem Cell Research Therapy 2013, 4:68 http://stemcellres.com/content/4/3/Page 5 of(Dako Cytomation, Glostrup, Denm.